Use of B-mode ultrasonography for sex determination and maturation monitoring in Prochilodus brevis (Steindachner 1875)

Carminda Sandra Brito Salmito-Vanderley, Pedro Emidio Leite Moraes Ferreira, Mirley Barbosa de Souza, Guilherme Melo Madeira, Bruna Farias Brito, Lívia Correia Magalhães, Carlos Henrique Sousa de Melo, Assis Rubens Montenegro

Resumo


The Brazilian bocachico is a rheophilic fish from the northeast region of Brazil that holds great economic and scientific interest. The absence of evident sexual dimorphism and the invasive character of sexing methods and evaluation of gonadal maturation are obstacles to its artificial reproduction. In this scenario, ultrasonography emerges as a non-invasive method for sexing. The objectives of this study were to establish an ultrasound sexing method for Brazilian bocachico and select equations to estimate the gonadosomatic index (GSI), gonad weight (GW), and gonad volume (GV) of live bocachico. A total of 32 bocachico fish (18 females and 14 males) were analyzed ultrasonographically and morphologically. The association of morphometric and ultrasonographic parameters was investigated using Pearson Correlations. The following prediction equations were selected using multiple regression analysis: GSI = 16.22 × transvarea; GW = 30.3 × transvarea; and GV = 0.001260036 × pixels. A model capable of predicting the sex of the animals based on the ultrasonographic parameters was obtained using partial least-squares discriminant analysis with principal component analysis. The significance level adopted was P < 0.05. In conclusion, ultrasonography is a useful tool to perform sexing and evaluate the gonads during the breeding season, contributing to artificial reproduction in captivity.

Palavras-chave


Fisheries. Brazilian Bocachico. Native fish. Gonadosomatic Index. Ultrasound.

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Referências


ALMEIDA-MONTEIRO, P. S. et al. Sperm vitrification of Prochilodus brevis using Powder Coconut Water (ACP-104) in association with different cryoprotectant concentrations. Aquaculture Research, v. 51, n. 11, p. 4565-4574, 2020.

BOMFIM, A. C. et al. Reproductive biology and variations in the gonadal development of the fish Curimatã (Prochilodus brevis Steindachner, 1875) in captivity. Biota Amazônica, v.5, p. 57-60, 2015.

BRYAN J. L.; WILDHABER, M. L.; NOLTIE D. B. Examining Neosho madtom reproductive biology using ultrasound and artificial photothermal cycles. North American Journal of Aquaculture, v. 67, n.3, p. 221-230, 2005.

CASTAÑEDA-CORTÉS, D. C.; FERNANDINO, J. I. Stress and sex determination in fish: from brain to gonads. The International Journal of Developmental Biology, v. 65, n.4-5-6, p. 207-214, 2021.

COLOMBIER, S.B. et al. Ultrasonography as a non-invasive tool for sex determination and maturation monitoring in silver eels. Fisheries Research, v. 164, p. 50-58, 2015.

CREPALDI, D.V. et al. A ultra-sonografia na piscicultura. Revista Brasileira de Reprodução Animal, v. 30, n. 3/4, p. 174-181, 2006.

GUITREAU, A. M. et al. Fish Handling and Ultrasound Procedures for Viewing the Ovary of Submersed, Nonanesthetized, Unrestrained Channel Catfish. North American Journal of Aquaculture, v. 74, p. 128-187, 2012.

JENKINS, M. R. et al. Natural and anthropogenic sources of habitat variation influence exploration behaviour, stress response, and brain morphology in a coastal fish. Journal of Animal Ecology, v. 90, n. 10, p. 2446-2461, 2021.

KAUFFOLD, J. et al. Principles and Clinical Uses of Real-Time Ultrasonography in Female Swine Reproduction. Animals, v. 9, n. 11, p. 1-17, 2019.

KUCHARCZYK, D. et al. Use of an ultrasound device to determine sex and to perform gonad biopsy in the European eel Anguilla Anguilla. Brazilian Journal Of Veterinary Research And Animal Science, v. 53, n. 2, p. 199-206, 2016.

MANTZIARAS, G.; LUVONI, G. C. Advanced ultrasound techniques in small animal reproduction imaging. Reproduction in Domestic Animals, v. 55, n. 2, p. 1-9.

MEDAN, M.S.; AEL-ATY, A. M. A. Advances in ultrasonography and its applications in domestic ruminants and other farm animals reproduction. Journal of Advanced Research, v. 1, n.2, p. 123-128.

NAEVE, I. et al. Ultrasound as a noninvasive tool for monitoring reproductive physiology in female Atlantic salmon (Salmo salar). Physiological reports, v. 6, p. e13640, 2018.

NAEVE, I. et al. Ultrasound as a noninvasive tool for monitoring reproductive physiology in male Atlantic salmon (Salmo salar). Physiological Reports, v. 7, n. 13, p. e14167.

NASCIMENTO, W.S. et al. Length–weight relationship for seven freshwater fish species from Brazil. Journal of Applied Ichthyology, v. 28, p. 272-274, 2012.

NOVELO, N.D.; TIERSCH, T. R. A review of the use of ultrasonography in fish reproduction. North American Journal of Aquaculture, v. 74, n.2, p.169–181, 2012.

PIRES, L.B. et al. Colossoma macropomum females can reproduce more than once in the same reproductive period. Animal Reproduction Science, v. 196, p. 138-142, 2018.

SHIMODA, E. D. et al. Influência da presença da fêmea sobre as características seminais do curimbatá (Prochilodus marggravii Walbaum, 1972). Revista Brasileira de Ciência Veterinária, v. 4, n. 1, p. 39-42, 2015.

TORRES, T. M. et al. Sperm cryopreservation of Prochilodus brevis using different concentrations of non-permeable cryoprotectants. Animal Reproduction, v.19, n.1, e20210083, 2022.

ZOHAR, Y. Fish reproductive biology – Reflecting on five decades of fundamental and translational research. General and Comparative Endocrinology, v. 300, p. 1-15, 2021.




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