Annatto seed by-product in diets containing sorghum for commercial laying hens

Marcelle Craveiro Abreu de Melo, Ednardo Rodrigues Freitas, Francisco Diego Teixeira Dantas, Danilo Rodrigues Fernandes, Pedro Henrique Watanabe


This study aimed to evaluate the effect of including annatto seed by-product (ASB) in diets containing sorghum on nutrient utilization, performance and characteristics of the eggs of laying hens. A total of 288 Lohman Brown laying hens, 34 weeks of age, were distributed in completely randomized design with six treatments and six replications of the eight birds each other. The treatments consisted of a diet with corn as an energy source and other with sorghum and inclusion of 0, 25, 45, 65 and 85 g/kg of ASB. Comparing the results, increasing levels of RSU did not affect the metabolizability of nutrients, and utilization the energy of the feed. It was also observed there was no effect on the performance and parameters egg quality, except for yolk color. There was a significant linear increase in yolk colour with inclusion of ASB. We concluded that the annatto seed by-product can be used at levels up to 85 g/kg in laying hen diets containing sorghum as the main source of energy, with the possibility of reducing yolk pigmentation problems, caused by the total substitution of corn by sorghum, with the inclusion of ASB from 25 g/kg.


Alternative feedstuff; Bixa orellana L;Bixin; Carotenoids; Yolk colour.

Texto completo:

PDF (English)


ARRAYA, H. H. et al. Composicion y empleo del achiote (Bixa orellana L.) en raciones para gallinas ponedoras, para la pigmentacion de la yema del huevo. Agronomía Costarricense. v.1, p.143-150, 1977.

BOLOGNESI, V. J.; GARCIA, C. E. R. Annatto carotenoids as additives replacers in meat products. In: GRUMEZESCU, A.; HOLBAN, A. M. Alternative and replacement foods. v.17. London: Elsevier Science, 2018. cap. 12, p.355-384.

BRAZ, N. M. et al. Semente residual do urucum na alimentação de poedeiras comerciais: desempenho e características dos ovos. Acta Scientiarum Animal Sciences. v.29, p.129-133, 2007.

FAGUNDES, N. S. et al. Replacing corn with sorghum in the diet alters intestinal microbiota without altering chicken performance. Journal of Animal Physiology and Animal Nutrition. v. 101, p. 371-382, 2017.

FREITAS, E. R. et al. Comparação de métodos de determinação da gravidade específica de ovos e poedeiras comerciais. Pesquisa Agropecuária Brasileira. v.39, p.509-512, 2004.

FREITAS, E. R. et al. Substituição do farelo de soja pelo farelo de coco em rações contendo farelo da castanha de caju para frangos de corte. Revista Brasileira de Zootecnia. v.40, p.1006-1013, 2011.

GARCIA, E. A. et al. Desempenho e qualidade dos ovos de poedeiras comerciais alimentadas com semente de Urucum (Bixa orellana L.) moída na dieta. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. v.16, p. 689-697, 2009.

GARCIA, E. A. et al. Ground annatto seeds (bixa orellana l.) in sorghum-based commercial layer diets and their effects on performance, egg quality, and yolk pigmentation. Brazilian Journal of Poultry Science. v.12, p.259-264, 2010.

GARCIA, R. G. et al. Desempenho e qualidade dos ovos de poedeiras alimentadas com semente de urucum. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR. v.18, p.17-20, 2015.

KUFEL, L.G.S. et al. Performance and egg quality of Japanese quails fed ground sorghum diets and increasing levels of Brazilian ginseng (Pfaffia paniculata). Livestock Science. v.227, p17–21, 2019.

LAGANÁ, C. et al. Turmeric root and annato seed in second-cycle layer diets: performance and egg quality. Brazilian Journal of Poultry Science. v.13, p.171-176, 2011.

MATTERSON, L. D. et al. The metabolizable energy of feeds ingredient for chickens. Agricultural Experiment Station Research Report. n.7, p. 3-11, 1965.

MOLNÁR, A. et al. Sequential and choice feeding in laying hens: adapting nutrient supply to requirements during the egg formation cycle. World's Poultry Science Journal. v.74, p199-210, 2018.

MORAES, C. A. et al. Performance and meat chemical composition of quails fed with different sorghum levels instead of corn. Ciência Rural. v. 46, p. 933-936, 2016.

MOURA, A. M. A. et al. Desempenho e qualidade do ovo de codornas japonesas alimentadas com rações contendo sorgo. Revista Brasileira de Zootecnia. v.39, p.2697-2702, 2010.

NATIONAL RESEARCH COUNCIL. Nutrient requirements of poultry. 9th ed. Washington, D.C., USA, 1994.

OFFICIAL METHODS OF ANALYSIS OF THE ASSOCIATION ANALYTICAL CHEMISTS. Official methods of analysis of AOAC International. 15th ed. Arlington, Virginia, USA,1995.

RADDATZ-MOTA, D. et al. Achiote (Bixa orellana L.): a natural source of pigment and vitamin E. Journal of Food Science and Technology. v.54, p.1729-1741, 2017.

ROJAS V.V.; CALLACNA C. M.; ARNAIZ P. V. Use of an additive canthaxanthin based and annatto extract in diets of laying hens and its effect on the color of the yolk and the egg shelf life. Scientia Agricola. v.6, p.191–199, 2015.

SALEH, A. A. et al. The effects of replacing corn with low-tannin sorghum in broiler’s diet on growth performance, nutrient digestibilities, lipid peroxidation and gene expressions related to growth and antioxidative properties. Journal of Applied Animal Research. v.47, p.532–539, 2019.

SIBBALD, I. R.; SLINGER, S. J. A biological assay for metabolizable energy in feed ingredients together with finding wich demonstrate some of the problems associated with the evaluation of fats. Poultry Science. v.42, p.313-325, 1963.

SILVA, J. H. V. Efeitos da inclusão do resíduo da semente de urucum (Bixa orellana L.) na dieta para de frangos de corte: desempenho e características de carcaça. Revista Brasileira de Zootecnia. v.34, p. 1606-1613, 2005.

SILVA, J. H. V. et al. Resíduo da semente de urucum (bixa orellana) como corante da gema, pele, bico e ovário de poedeiras avaliado por dois métodos analíticos. Ciência e Agrotecnologia. v.30, p.988-994, 2006.

SOUZA, D.H. et al. Inclusion of annatto seed by-product in diets containing sorghum for slow-growth broilers. Ciência e Agrotecnologia. v.39, p.248-259, 2015.

SOUZA, L. A. Z. et al. Egg yolk colour and retinol concentration of eggs from laying hens fed diets containing carrot and beetroot meal. Czech Journal of Animal Science. v.64, p.395–403, 2019.

SPADA, F. P. et al. Adição de carotenóides naturais e artificiais na alimentação de galinhas poedeiras: efeitos na qualidade de ovos frescos e armazenados. Ciência Rural. v.42, p.346-353, 2012.

UL-ISLAM, S.; RATHER, L. J.; MOHAMMAD, F. Phytochemistry, biological activities and potential of annatto in natural colourant production for industrial applications: a review. Journal of Advanced Research. v.7, p.499–514, 2015.

VERA, V. R; CUSTODIO, M. C.; PERALES, V. A. Uso de un aditivo a base de cantaxantina y extracto de achiote en dietas de gallinas de postura y su efecto sobre la coloración de la yema y la vida de anaquel del huevo. Science in Agrochemicals. v.6, p.191-199, 2015.

Revista Ciência Agronômica ISSN 1806-6690 (online) 0045-6888 (impresso), Site:, e-mail: - Fone: (85) 3366.9702 - Expediente: 2ª a 6ª feira - de 7 às 17h.