Nutritional quality of the protein of Vigna unguiculata L. Walp and its protein isolate

Karoline de Macêdo Gonçalves Frota, Lays Arnaud Rosal Lopes, Izabel Cristina Veras Silva, José Alfredo Gomes Arêas

Resumo


A protein’s true digestibility and amino acid composition are important characteristics for its nutritional characterisation. This study determined the true digestibility of the protein from cowpea (Vigna unguiculata L. Walp.) and of its protein isolate, and their nutritional values were estimated after correcting for the amino acid score. The protein was isolated from the defatted whole bean by its alkaline solubilisation and isoelectric precipitation. The amino acid composition of the protein from the whole bean and from the isolate was determined by ion-exchange chromatography. Protein digestibility was assessed by the nitrogen balance method. The amino acid score is the lowest value obtained from the ratio between the individual amount of each essential amino acid present within the protein and the recommendation of each one for preschool infants. Methionine was the limiting amino acid in both the whole bean and the isolate. The true digestibility of the protein corrected by the amino acid score is an estimate of its nutritional value. The amino acid score for the whole bean was 0.44, and protein digestibility was 86.7%. For the isolate, these values were 0.60 and 96.7%, respectively. Correcting each score by the digestibility resulted in an estimated nutritive value of 38% and 58% for the whole bean and the isolate, respectively. Therefore, by having higher digestibility and increasing the bioavailability of essential amino acids, cowpea protein isolate is of interest for inclusion in food products, especially cereal-based products, which contain lysine as a limiting amino acid and are rich in methionine. 


Palavras-chave


Legume; Amino acid composition; Proteins; Digestibility

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Referências


ANDRADE, F. N. et al. Estimativas de parâmetros genéticos em genótipos de feijão-caupi avaliados para feijão fresco. Revista Ciência Agronômica, v. 41, n. 2, p. 253-258, 2010.

ANJOS, F. et al. Chemical composition, amino acid digestibility, and true metabolizable energy of cowpeas as affected by roasting and extrusion processing treatments using the cecectomized rooster assay. The Journal of Applied Poultry Research, v. 25, n. 1, p. 85-94, 2016.

ANYANGO, J. O.; KOCK, H. L. de; TAYLOR; J. R. N. Impact of cowpea addition on the protein digestibility corrected amino acid score and other protein quality parameters of traditional African foods made from non-tannin and tannin sorghum. Food Chemistry, v. 124, n. 3, p. 775-780, 2011.

ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. Washington, 2007.

BOYE, J.; WIJESINHA-BETTONI, R.; BURLINGAME, B. Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method. The British Journal of Nutrition, v. 108, n. S2, p. S183-S211, 2012.

CERVANTES-PAHM, S. K.; LIU, Y.; STEIN, H. H. Digestible indispensable amino acid score and digestible amino acids in eight cereal grains. British Journal of Nutrition, v. 111, n. 9, p. 1663-1672, 2014.

CRUZ, G. A. D. R. et al. Comparação entre a digestibilidade protéica in vitro e in vivo de diferentes cultivares de feijão (phaseolus vulgaris l.) armazenadospor 30 dias. Alimentos e Nutrição Araraquara, v. 16, n. 3, p. 265-271, 2005.

DAMADORAN, S. Aminoácidos, peptideos e proteinas. In: DAMCIDARAN, S.; PARKIN, K. L.; FENNEMA, O. R. Química de Alimentos de Fennema. PortoAlegre: Arlmad, 2010. cap. 5, p. 180-260.

ELHARDALLOU, S. et al. Amino acid composition of cowpea (Vigna ungiculata L. Walp) flour and its protein isolates. Food and Nutrition Sciences, v. 6, n. 9, p. 790-797, 2015.

FOOD AND AGRICULTURE ORGANIZATION; WORLD HEALTH ORGANIZATION. Protein quality evaluation. Roma, 1991. 66 p. (FAO Food and Nutrition Paper, 51).

FERY, R. L.; SINGH, B. B. Cowpea genetics: a review of the recent literature. In: SINGH, B. B. et al. (Ed.) Advances in cowpea research. Ibadan: IITA: JIRCAS, 1997. cap. 2, p. 13-15.

FREIRE FILHO, F. R. et al. Feijão-caupi no Brasil: produção, melhoramento genético, avanços e desafios. Teresina: Embrapa Meio-Norte, 2011. 84 p.

FROTA, K. M. G. et al. Cholesterol-lowering properties of whole cowpea seed and its protein isolate in hamsters. Journal of Food Science, v. 73, n. 9, p. H235-H240, 2008.

IQBAL, A. et al. Nutrition quality of important food legumes. Food ChemistrY, v. 97, n. 2 p. 331-335, 2006.

KALPANADEVI, V.; MOHAN, V. R. Effect of processing on antinutrients and in vitro protein digestibility of the underutilized legume, Vigna unguiculata (L.) Walp subsp. unguiculata. LWT-Food Science and Technology, v. 51, n. 2, p. 455-461, 2013.

KHATTAB, R. Y.; ARNTFIELD, S. D. Nutritional quality of legume seeds as affected by some physical treatments 2. Antinutritional factors. LWT-Food Science and Technology, v. 42, n. 6, p. 1113-1118, 2009.

MARQUES, M. R. et al. Proteolytic hydrolysis of cowpea proteins is able to release peptides with hypocholesteerolemic activity. Food Research International, v. 77, Special Issue, Part 1, p. 43-48, 2015.

MOORE, S; SPACKMAN, D. C.; STEIN, W. H. Automatic recording apparatus for use in the chromatography of amino acids. Federation proceedings, v. 17, n. 4, p. 1107-1115, 1958.

OLFERT, E.; CROSS, B. M.; MCWILLIAM, A. A. Guide to the care and use of experimental animal. 2. ed. Canadá: Canadian Council on Animal Care, 1993. v. 1 .

PANDURANGAN, S. et al. Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition. Frontiers in Plant Science, v. 6, n. 92, 2015.

PEREIRA, C. A. S.; COSTA, N. M. B. Proteínas do feijão preto sem casca: digestibilidade em animais convencionais e isentos de germes (germ-free). Revista de Nutrição, v. 15, n. 1, p. 5-14, 2002.

PIRES, C. V. et al. Qualidade nutricional e escore químico de aminoácidos de diferentes fontes proteicas. Ciência e Tecnologia de Alimentos, v. 26, n. 1, p. 179-187, 2006.

RANGEL, A. et al. Biological evaluation of a protein isolate from cowpea (Vigna unguiculata) seeds. Food Chemistry, v. 87, n. 4, p. 491-499, 2004.

REEVES, P. G.; NIELSEN, F. H.; TAHEY, G. C. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc writing committee on the reformulation of the AIN-76A rodent diet. Journal of Nutrition, v. 123, n. 11, p. 1939-1951, 1993.

RUSSO, R.; REGGIANI, R. Evaluation of protein concentration, amino acid profile and antinutritional compounds in hempseed meal from dioecious and monoecious varieties. American Journal of Plant Sciences, v. 6, n. 1, p. 14-22, 2015.

RUTHERFURD, S. M. et al. Protein digestibility-corrected amino acid scores and digestible indispensable amino acid scores differentially describe protein quality in growing male rats. The Journol of Nutrition, v. 145, n. 2, p. 372-379, 2014.

RUTHERFURD, S. M.; BAINS, K; MOUGHAN, P. J. Available lysine and digestible amino acid contents of proteinaceous foods of India. British Journal of Nutrition, v. 108, n. 2, p. 59-68, 2012.

SARWAR, G.; XIAO, C.; COCKELL, K. A. Impact of antinutritional factors in food proteins on the digestibility of protein and the bioavailability of amino acids and on protein quality. The British Journal of Nutrition, v. 108, n. S2, p. S315-S332, 2012.

SHAHEEN, N. et al. Amino acid profiles and digestible indispensable amino acid scores of proteins from the prioritized key foods in Bangladesh. Food Chemistry, v. 213, n. 15, p. 83-89, 2016.

SILVA, A. C. C.; FROTA, K. M. G.; ARÊAS, J. A. G. Função plenamente reconhecida de nutrientes: proteínas. São Paulo: ILSI, 2012. v. 20, p. 1-15.

SPIES, J. R. Determination of tryptophan in protein. Analytical Chemistry, v. 39, n. 9, p. 1412-1415, 1967.

VASCONCELOS, I. M. et al. Protein fractions, amino acid composition and antinutritional constituents of high-yielding cowpea cultivars. Journal of Food Composition and Analysis, v. 23, n. 1. p. 54-60, 2010.

VILLARINO, C. B. J. et al. The effects of lupin (Lupinus angustifolius) addition to wheat bread on its nutritional, phytochemical and bioactive composition and protein quality. Food Research International, v. 76, Part. 1, p. 58-65, 2014.

WRIGHT, D. J.; BUMSTEAD, M. R. Legume proteins in food technology. Philosophical Transactions of the Royal Society of London, v. 304, n. 1120, p. 381-393, 1984.




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