Bukholderia strains promote Mimosa spp. growth but not Macroptilium atropurpureum

Kaliane Sírio Araújo, Fernanda de Carvalho, Fatima Maria de Souza Moreira

Resumo


The aim of this study was to evaluate the relationship and symbiotic efficiency of 14 strains of Burkholderia isolated from rupestrian grasslands, using M. atropurpureum and Mimosa tenuiflora as trap plants, with the species M. atropurpureum, Mimosa bimucronata and M. foliolosa. For the nodulation and symbiotic efficiency test in M. atropurpureum, long-neck bottles containing nutrient solution were used. The experiments with Mimosa spp. were carried out in tubes containing vermiculite (160 cm3) and sand (80 cm3) (2:1). The parameters under evaluation were number of nodules, nodules dry matter production, shoots dry matter, roots dry matter, and total dry matter production for all the species analyzed; and plant height, diameter, and the Dickson quality index for Mimosa species. Of the 14 tested strains, two nodulated M. atropurpureum; however, they were ineffective in promoting plant growth. All the tested strains established symbiosis with M. bimucronata, and 12 strains nodulated M. foliolosa. Of these, six promoted growth in M. bimucronata, and seven presented symbiotic efficiency in M. foliolosa. The strains UFLA 01-739, UFLA 01-748 and UFLA 01-751, isolated from M. tenuiflora, and UFLA 04-260 and UFLA 04-405, isolated from M. atropurpureum, stood out as potential inoculants for the Mimosa species evaluated in this study.


Palavras-chave


Rupestrian grasslands; Nitrogen-fixing bacteria; Symbiosis; Mimosa bimucronata; Mimosa foliolosa

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Referências


ANGUS, A. A. et al. Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a nodulating and plant growth promoting beta-proteobacterium, are influenced by environmental factors. Plant and Soil, v. 369, n. 1, p. 543-562, 2013.

BARRET, C. F.; PARKER, M. A. Coexixtence of Burkholderia, Cupriavidus and Rhizobium sp. nodule bacteria on two Minosa spp. in Costa Rica. Applied and Environmental Microbiology, v. 72, n. 2, p. 1198-12016, 2006.

BARRET, C. F.; PARKER, M. A. Prevalence of Burkholderia sp. nodule symbionts on four mimosoid legumes from Barro Colorado Island, Panama. Systematic and Applied Microbiology, v. 28, n. 1, p. 57-65, 2005.

BONTEMPS, C. et al. Burkholderia species are ancient symbionts of legumes. Molecular Ecology, v. 19, n. 1, p. 44-52, 2010.

CARVALHO, F. Abundância de espécies de plantas e diversidade de simbiontes radiculares em campos rupestres da serra do cipó, MG. Tese (Doutorado em Ecologia, Conservação e Manejo da Vida Silvestre) - Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, 2010.

CARVALHO, F. et al. Relationship between physical and chemical soil attributes and plant species diversity in tropical mountain ecosystems from Brazil. Journal of Mountain Science, v. 11, n. 4, p. 875-883, 2014.

CARVALHO, F. et al. The mosaico f habitats in the high-altitude brazilian rupestrian fields is a hotspot for arbuscular mycorrhizal fungi. Applied Soil Ecology, v. 52, n. 1, p. 9-19, 2012.

CHAER, G. M. et al. Nitrogen-fixing legume tree species for the reclamation of severely degraded lands in Brasil. Tree Physiology, v. 31, p. 139-149, 2011.

CHEN, W. M. et al. Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella. International Journal of Systematic and Evolutionary Microbiology, v. 57, n. 5, p. 1055-1059, 2007.

CHEN, W. M. et al. Burkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia. International Journal of Systematic and Evolutionary Microbiology, v. 58, n. 9, p. 2174-2179, 2008.

CHEN, W. M. et al. Legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature. Journal of Bacteriology, v. 185, n. 24, p. 7266-7272, 2003.

CHEN, W. M. et al. Proof that Burkholderia forms effective symbioses with legumes: a study of novel Mimosa-nodulating strains from South America. Applied and Environmental Microbiology, v. 71, n. 11, p. 7461-7471, 2005.

CHEN, W.-M. et al. Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. From Taiwan and South America. International Journal of Systematic and Evolutionary Microbiology, v. 56, n. 8, p. 1847-1851, 2006.

COENYE, T.; VANDAMME, P. Diversity and significance of Burkholderia species occupying diverse ecological niches. Environmental Microbiology, v. 5, n. 9, p. 719-729, 2003.

DICKSON, A.; LEAF, A. L.; HOSNER, J. F. Quality appraisal of white spruce and white pine seedling stock in nurseries. Forest Chronicle, v. 36, n. 1, p. 10-13, 1960.

ELLIOTT, G. N. et al. Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum. Annals of Botany, v. 100, n. 7, p. 1403-1411, 2007.

FARIA, S. M. et al. Obtenção de estirpes de rizóbio eficientes na fixação biológica de nitrogênio para espécies florestais. Seropédica: Embrapa Agrobiologia, 1997. p. 1-4. (Recomendação Técnica, 3).

FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e agrotecnologia, v. 35, n. 6, p. 1039-1042, 2011.

FERREIRA, P. A. A. et al. Efficient nitrogen-fixing Rhizobium strains isolated from amazonian soils are highly tolerant to acidity and aluminium. World Journal of Microbiology and Biotechnology, v. 28, n. 5, p. 1947-1959, 2012.

FLORENTINO, L. A. et al. Sesbania virgata stimulates the occurrence of its microsymbiont in soils but does not innibit microsymbiont of other species. Scentia Agricola, v. 66, n. 5, p. 667-676, 2009.

FRED, E. B.; WAKSMAN, S. A. Laboratory manual of general microbiology. New York: McGraw-Hill Book, 1928. 143 p.

GYANESHWAR, P. et al. Legume-nodulating betaproteobacteria: diversity, host range, and future prospects. The American Phytopathological Society, v. 24, n. 11, p. 1276-1288, 2011.

HOAGLAND, D. A.; ARNON, D. D. The water-culture method for growing plants without soil. Berkeley: California Agricultural of Experiment Station, 1950. 32 p. (Circular 347).

REIS JÚNIOR, F. B. dos et al. Nodulation and nitrogen fixation by Mimosa spp in the Cerrado and Caatinga biomes of Brazil. New Phytologist, v. 186, n. 4, p. 934-946, 2010.

LIMA, A. S. et al. Nitrogen-fixing bacteria communities occurring in soils under different uses in the Western Amazon Region as indicated by nodulation of siratro (Macroptilium atropurpureum). Plant and Soil, v. 319, n. 1, p. 127-145, 2009.

MISHRA, R. P. N. et al. Genetic diversity of Mimosa pudica rhizobial symbionts in soils of French Guiana: investigating the origin and diversity of Burkholderia phymatum and other beta-rhizobia. Microbiology Ecology, v. 79, n. 2, p. 487–503, 2012.

MOULIN, L. et al. Nodulation of legumes by members of the b-subclass of Proteobacteria. Nature, v. 411, n. 6840, p. 948-950, 2001.

NEGREIROS, D.; MORAIS, M. L. B.; FERNANDES, G. W. Caracterização da fertilidade dos solos de quatro leguminosas de campos rupestres, serra do cipó, MG, Brasil. Revista de la Ciência del Suelo y Nutrición Vegetal, v. 8, n. 3, p. 30-39, 2008.

SILVA, K. et al. Diazotrophic Burkholdria species isolated from the Amazon region exhibit phenotypical, functional andd genetic diversity. Systematic and Apllied Microbiology, v. 35, n. 4, p. 253-262, 2012

SOARES, B. L. et al. Cowpea symbiotic efficiency, pH and aluminum tolereance in nitrogen-fixing bactéria. Scientia Agricola, v. 71, n. 3, p. 171-180, 2014.

TALBI, C. et al. Burkholderia phymatum strains capable of nodulating Phaseolus vulgaris are present in Moroccan soils. Applied and Environmental Microbiology, v. 76, n. 13, p. 4587-4591, 2010.




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