Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño

Mauricio Reginaldo Alves dos Santos, Carolina Augusto de Souza, Eloísa Santana Paz

Resumo


The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuum var. annuum cv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combination. Percentage of callus induction and the explant area covered by callus cells (ACCC) were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The highest callus induction percentage and ACCC were observed with the combination of 0.5 mg L–1 BA + 4.0 mg L–1 2,4-D for leaf and internodal explants and 0.5 mg L–1 BA + 4.0 mg L–1 2,4-D for nodal explants. The calluses produced were friable and whitish, and their growth pattern followed a sigmoid shape. The deceleration phase started on the 31st day of cultivation for leaf explants, on the 26th for internodal explants, and on the 29th day for nodal explants.

Palavras-chave


Fisiologia Vegetal; Biotecnologia; Produtos Naturais

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Referências


ARROWSMITH, S. et al. Effects of salt stress on capsaicin content, growth, and fluorescence in a Jalapeño cultivar of Capsicum annuum (Solanaceae). BIOS, v. 1, n. 83, p. 1-7, 2012.

BALBUENA, T. S. et al. In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae). Acta Botanica Brasilica, v. 23, n. 1, p. 274-281, 2009.

BARBOSA, M. H. P. et al. Effect of growth regulators and different explants on in vitro morphogenesis of Capsicum annuum L. Ciência rural, v. 24, n. 1, p. 67-72. 1994.

BIANCHETTI, L. de B. Aspectos morfológicos, ecológicos e biogeográficos de dez táxons de Capsicum (Solanaceae) ocorrentes no Brasil. 1996. 174 f. Dissertação (Mestrado em Botânica) - Universidade de Brasília, Brasília, 1996.

CASTRO, A. H. F. et al. Curva de crescimento, atividade da fenilalanina amônia-liase e teores de fenóis e taninos totais em calos de Stryphnodendron adstringens (Mart.) Coville (Fabaceae-Mimosoideae). Plant Cell Culture & Micropropagation, v. 4, n. 2, p. 99-104, 2008.

CORDEIRO, I. M. C. C. et al. Indução de calos in vitro de Paricá (Schizolobium amazonicum Huber ex Ducke). Plant Cell Culture & Micropropagation, v. 3, n. 1, p. 35-40, 2007.

DALPONTE, F.; VALLE, J. A. B.; VALLE, R. C. S. C. Uso de adsorventes de compostos fenólicos e diferentes explantes na produção de massa celular. Dynamis, v. 15, n. 2, p.12-17, 2010.

EBIDA, A. I. A.; HU, C. In vitro morphogenetic responses and plant regeneration from pepper (Capsicum annuum L. cv. Early California Wonder) seedling explants. Plant Cell Reports, v. 13, n. 2, p. 107-110, 1993.

FEITOSA, L. S. et al. Indução e análise histológica de calos em explantes foliares de Jatropha curcas L.(Euphorbiaceae). Bioscience Journal, v. 29, n. 2, p. 370-377, 2013.

GERSHENZON, J.; ENGELBERTH, J. E. Metabólitos secundários e defesa vegetal. In: TAIZ, L.; ZEIGER, E. Fisiologia Vegetal. 5. ed. Porto Alegre: Artmed, 2013. cap. 13, p. 369-399.

HUSSAIN, M. S. et al. Current approaches toward production of secondary plant metabolites. Journal of Pharmacy & BioAllied Sciences, v. 4, n. 1, p. 10-20, 2012.

KHAN, H. et al. In vitro organogenesis from internode derived callus cultures of Capsicum annuum L. Journal of Plant Biochemistry and Biotechnology, v. 20, n. 1, p. 84-89, 2011.

LUZ, F. J. F. Caracterizações morfológica e molecular de acessos de pimenta (Capsicum chinense Jaqc.). 2007. 70 f. Tese (Doutorado em Produção Vegetal) - Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, São Paulo, 2007.

MENDONÇA, E. G. et al. Genetic transformation of Eucaliptus camaldulensis by agrobalistic method. Revista Árvore, v. 37, n. 3, p. 419-429, 2013.

MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v. 15, n. 3, p. 473-497, 1962.

MURTHY, H. N.; LEE, E.-J.; PAEK, K.-Y. Production of secondary metabolites from cell and organ culture: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture, v. 118, n. 1, p. 1-16, 2014.

NOGUEIRA, R. C. et al. Curva de crescimento e análises bioquímicas de calos de murici-pequeno (Byrsonima intermedia A. Juss.). Revista Brasileira de Plantas Medicinais, v. 10, n. 1, p. 44-48, 2008.

OTROSHY, M.; MORADI, K.; KHAYAM NEKOUEI, M. The effect of different cytokinin in propagation of Capsicum annuum L. by in vitro nodal cutting. Trakia Journal of Sciences, v. 9, n. 3, p. 21-30, 2011.

PEIXOTO, C. P.; CRUZ, T. V.; PEIXOTO, M. F. S. Análise quantitativa do crescimento de plantas: conceitos e prática. Enciclopédia Biosfera, v. 7, n. 13, p. 51-76, 2011.

POZO, J. C. D. et al. Hormonal control of the plant cell cycle. Biologia Plantarum, v. 123, n. 2, p. 173-183, 2005.

RICCI, N. et al. Seed quality of Jalapeño pepper according to fruit maturation and post harvest rest. Pesquisa Agropecuária Tropical, v. 43, n. 2, p. 123-129, 2013.

SANATOMBI, K.; SHARMA, G. J. Capsaicin content and pungency of different Capsicum spp. cultivars. Notulae Botanicae Horti Agrobotanici, v. 36, n. 2, p. 89-90, 2008.

SANTIAGO, E. J. A. Caracterização morfológica e bioquímica de calos de pimenta longa (Piper hispidinervium Candolle, De Candolle). 2003. 162 f. Tese (Doutorado em Fitotecnia) - Departamento de Fitotecnia, Universidade Federal de Lavras, Lavras, 2003.

SANTOS, C. G. et al. Indução e análise bioquímica de calos obtidos de segmentos foliares de Coffea arabica L., cultivar Rubi. Ciência e Agrotecnologia, v. 27, n. 3, p. 571-577, 2003.

SANTOS, M. R. A. et al. Callogenesis in leaves of Kalanchoe pinnata Lam. by 2,4-D and BA action. Revista Brasileira de Plantas Medicinais, v. 16, n. 3, p. 760-764, 2014. Suplemento 1.

SANTOS, M. R. A.; SOUZA, C. A. Dedifferentiation of leaf cells and growth pattern of calluses of Capsicum annuum cv. Etna. Australian Journal of Basic and Applied Sciences, v. 10, n. 12, p. 362-368, 2016.

SANTOS, M. R. A; FERREIRA, M. G. R.; SARUBO, V. Determination of callus growth curve in conilon coffee. Revista Caatinga, v. 23, n. 1, p. 133-136, 2010.

SINGH, J.; TIWARI, K. N. In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L. - an important medicinal plant. Industrial Crops and Products, v. 35, n. 1, p. 224-229, 2012.

SOUZA, J. M. M. et al. Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis. Biologia Plantarum, v. 55, n. 4, p. 703-307, 2011.

SOUZA, J. M. M.; BERKOV, S.; SANTOS, A. S. Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS. Anais da Academia Brasileira de Ciências, v. 86, n. 3, p. 1015-1027, 2014.

UMAMAHESWARI, A.; LALITHA, V. In vitro effect of various growth hormones in Capsicum annuum L. on the callus induction and production of capsaicin. Journal of Plant Sciences, v. 2, n. 5, p. 545-551, 2007.

VALLE, R. de C. S. C. Estratégias de cultivo de células de pimenta longa (Piper hispidinervium) e determinação de parâmetros cinéticos. 2003. 165 f. Tese (Doutorado em Engenharia Química) - Centro Tecnológico, Universidade Federal de Santa Catarina, Florianópolis, 2003.

VANISREE, M. et al. Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Botanical Bulletin of Academia Sinica, v. 45, n. 1, p. 1-22, 2004.




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