The potential of Mythimna sequax Franclemont eggs for the production of Trichogramma spp. after cryopreservation in liquid nitrogen

Magda Fernanda Paixão, Luís Amilton Foerster, Marion do Rocio Foerster

Resumo


The cryopreservation of noctuid eggs in liquid nitrogen has proved be a promising tool in the mass production of Trichogramma, however studies into this technique have only just begun. This study evaluated the response of different densities of the female of Trichogramma pretiosum Riley to the parasitism of Mythimna sequax eggs stored and not stored in liquid nitrogen, and the performance of females reared only in cryopreserved eggs. The study evaluated the influence of the number of T. pretiosum females (4, 8 and 12) released to parasitise 40 M. sequax eggs, stored and not stored for 15 days in liquid nitrogen, as well as the performance of T. pretiosum females reared in eggs stored for three generations and females reared in non-stored eggs. Parasitism by T. pretiosum in stored eggs was 84%, twice the value obtained in previous studies. The emergence of parasitoids was greater than 95% in both experiments. The performance of females raised in stored eggs did not differ from that of females raised in non-stored eggs. The data show that the technique of cryopreservation of M. sequax eggs may be a viable alternative in the mass production of T. pretiosum.


Palavras-chave


Egg storage; Ultra-low temperatures; Noctuids; Egg parasitoids

Texto completo:

PDF

Referências


avanci, m. do r. F.; foerster, l. a. composição de lípidios em ovos de duas espécies de hospedeiros de Trichogramma pretiosum (Hymenoptera: Trichogramatidae) e sua relação com a tolerância a baixas temperaturas. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife. Anais… Recife: UFPe, 2006. 1 CD.

BAI, B. et al. The effect of host size on quality attributes of the egg parasitoid Trichogramma pretiosum. Entomologia Experimentalis et Applicata, v. 64, p. 37-48, 1992.

BESERRA, E. B. et al. Características biológicas de linhagens de Trichogramma pretiosum desenvolvidas em ovos de Spodoptera frugiperda. Acta Scientiarum Agronomy, v. 25, n. 2, p. 479-483, 2003.

Castro, S. V. de et al. Agentes crioprotetores intracelulares: características e utilização na criopreservação de tecido ovariano e oócitos. Acta Scientiae Veterinariae, v. 39, n. 2, p. 957, 2011.

COBERT, S. A. Insect chemosensory responses: a chemical legacy hypothesis. Ecological Entomology, v. 10, p. 143-153, 1985.

CÔNSOLI, F. L.; KITAJIMA, E. W.; PARRA, J. R. P. Ultraestructure of the natural and factitious host eggs of Trichogramma galloi Zucchi and Trichogramma pretiosum Riley (Hym., Trichogrammatidae). Journal of Insect Morphology Embryology, v. 28, p. 211-229, 1999.

Corrêa-Ferreira, B. S.; Oliveira, M. C. N. Viability of Nezara viridula (L.) eggs for parasitism by Trissolcus basalis (Woll.), under different storage techniques in liquid nitrogen. Anais da Sociedade Entomologica do Brasil, v. 27, p. 101-107, 1998.

DOETZER, A. K.; FOERSTER, L. A. Storage of pentatomid eggs in liquid nitrogen and dormancy of Trissolcus basalis (Wollaston) and Telenomus podisi Ashmead (Hymenoptera: Platygastridae) adults as a method of mass production. Neotropical Entomology, v. 42, p. 534-538, 2013.

Durocher-Granger, L. et al. Gamete number and size correlate with adult size in the egg parasitoid Trichogramma euproctidis. Entomologia Experimentalis et Applicata, v. 140, n. 3, p. 262-268, 2011.

FAVETTI, B. M.; BUTNARIU, A. R.; DOETZER, A. K. Storage of Euschistus heros eggs (Fabricius) (Hemiptera: Pentatomidae) in liquid nitrogen for parasitization by Telenomus podisi Ashmead (Hymenoptera: Platygastridae). Neotropical Entomology, v. 43, p. 291-293, 2014.

GRECO, C. F.; STILINOVIC, D. Parasitization performance of Trichogramma spp. (Hym., Trichogrammatidae) reared on eggs of Sitotraga cerealella Oliver (Lep., Gelechidae), stored at freezing and subfreezing conditions. Journal of Applied Entomology, v. 122, p. 311-314, 1998.

JALALI, S. K.; SINGH, S. P. Diferential response of four Trichogramma species to low temperatures for short term storage. Entomophaga, v. 37, n. 1, p. 159-165, 1992.

Krechemer, F. S., Foerster, L. A. Mass production of Trichogramma spp. using Mythimna sequax eggs stored in liquid nitrogen. BioControl, v. 61, n. 5, p. 497-505, 2016.

LOHMANN, T. et al. Viabilidade do armazenamento de ovos de Anagasta kuehniella, Zeller (Lepidoptera: Pyralidae) em nitrogênio líquido para a produção de Trichogramma pretiosum, Riley (Hymenoptera: Trichogrammatidae). Revista Brasileira de Agroecologia, v. 2, p. 1551-1555, 2007.

MARCHIORO, C. A.; FOERSTER, L. A. Performance of the wheat armyworn, Mythimna sequax Franclemont, on natural and artificial diets. Neotropical Entomology, v. 41, n. 4, p. 288-295, 2012.

MILWARD-DE-AZEVEDO, E. M. V. et al. Desempenho reprodutivo de Nasonia vitripennis Walker (Hymenoptera: Pteromalidae) em pupas crioconservadas de Chrysomya megacephala Fabricius (Diptera: Calliphoridae): avaliação preliminar. Ciência Rural, v. 34, n. 1, p. 207-211, 2004.

MOREIRA, M. D. et al. Parasitismo e superparasitismo de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) em ovos de Sitotroga cerealella (Oliver) (Lepidoptera: Gelechiidae). Neotropical Entomology, v. 38, n. 2, p. 237-242, 2009.

NAVARRO, M. A. Trichogramma spp.: producción, uso y manejo en Colombia. Guadalajara de Buga: Imprectec, 1998, 176 p.

ÖZDER, N. Effect of different cold storage periods on parasitization performance of Trichogramma cacoeciae (Hymenoptera, Trichogrammatidae) on eggs of Ephestia kuehniella (Lepidoptera, Pyralidae). Biocontrol Science and Technology, v. 14, n. 5, p. 441-447, 2004.

PARON, M. J. F. O.; Ciociola, A. I.; Cruz, I. Resposta de Trichogramma atopovirilia Oatman & Platner (Hymenoptera: Trichogrammatidae) a diferentes densidades de ovos do hospedeiro natural, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). Anais da Sociedade Entomologica do Brasil, v. 27, p. 427-433, 1998.

PARRA, J. R. P. Técnicas de criação de Anagasta kuehniella, hospedeiro alternativo para produção de Trichogramma. In: PARRA, J. R. P.; ZUCCHI, R.A. (Ed.). Trichogramma e o controle biológico aplicado. Piracicaba: Fealq, 1997. p. 121-150.

PEREIRA, F. F. et al. Biologia e exigências térmicas de Trichogramma pretiosum Riley e T. exiguum Pinto & Platner (Hymenoptera: Trichogrammatidae) criados em ovos de Plutella xylostella (L.) (Lepidoptera: Plutellidae). Neotropical Entomology, v. 33, p. 231-236, 2004.

PITCHER, S. A. et al. Cold storage of Trichogramma ostrinae reared on Sitotroga cerealella eggs. BioControl, v. 47, p. 25-535, 2002.

Pratissoli, D. et al. Influência da densidade de ovos de Spodoptera frugiperda em alguns aspectos biológicos de três espécies de Trichogramma. Revista Brasileira de Milho e Sorgo, v. 4, p. 1-7, 2005.

SALT, G. The effects of hosts upon their insect parasites. Biological Reviews, v. 16, p. 239-263, 1941.

TEZZE, A. A.; BOTTO, E. N. Effect of cold storage on the quality of Trichogramma nerudai (Hymenoptera: Trichogrammatidae). Biological Control, v. 30, p. 11-16, 2004.

ZAMONER, M. Efeito do volume de ovos hospedeiros sobre o desenvolvimento, capacidade de parasitismo e longevidade de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae). 2005. 44 f. Dissertação (Mestrado em Zoologia) - Curso de Pós-Graduação em Zoologia, Universidade Federal do Paraná, Curitiba, 2005.




Revista Ciência Agronômica ISSN 1806-6690 (online) 0045-6888 (impresso), Site: www.ccarevista.ufc.br, e-mail: ccarev@ufc.br - Fone: (85) 3366.9702 - Expediente: 2ª a 6ª feira - de 7 às 17h.